The second STM report has been evaluated from the 2019-2020 call. Thibault Le Gratiet from ANSES Ploufragan-Plouzané-Niort visited Fabrizio Anniballi, Concetta Scalfaro and Bruna Auricchio from the Istituto Superiore di Sanità (ISS), Rome in Italy from 23rd of September till the 1st of October 2019 to learn 'Skills transfer regarding Multi Loci VNTR analysis, High resolution melting analysis and Whole Genome Sequencing analysis of Group III Clostridium botulinum' .

OBJECTIVE: Knowledge and skills transfer regarding Multi Loci VNTR analysis, High resolution melting analysis and Whole Genome Sequencing analysis of Group III Clostridium botulinum.

REPORT: The first objective of this short-term mission was to be trained for multi loci VNTR analysis (MLVA) for comparison of Clostridium botulinum group III, from DNA extraction to the analysis of capillary electrophoresis results. This method has been developed and validated by the Italian National Reference Center for Botulism (CNRB) at ISS during the ANIBOTNET project (2016-2019), where ISS and ANSES are partners. 

Training to all MLVA steps was provided during the STM using various DNA samples from ISS collection. Data
analysis was performed using Bionumerics. An ISS ongoing project of a new group III Clostridium botulinum typing method using High resolution melting (HRM) was also presented and thanks to this mission a collaboration between ANSES and ISS is under implementation to validate this method.
Last part of the mission was the initiation of the analysis of whole genome sequencing data using ARIES (Advanced Research Infrastructure for Experimentation in Genomics) Platform. Single Nucleotide Polymorphism (SNP) analysis of Group III C. botulinum using sequences from ISS collection was in particular demonstrated. Tutorials and recommendations were provided by ISS Finally, ISS scientists have also provided their scientific and technical expertise regarding certain specificities of C.botulinum group III, such as DNA extraction that can be challenging to obtain high quality DNA prior to sequencing.
This STM highlights the beneficial role of the Med-Vet-Net Association to enhance collaboration between institutes.